This colony has been slowly declining in population for months now. It looks like it's mainly just an excessive amount of deaths. I have no idea why this would be happening, and I even moved them all into a freshly cleaned container, but they continue to die. Right now the colony is down to about 150 workers. For the longest time I had two test tubes in there because they refused to move out of the old one, and instead just moved into both. Finally the other day I dumped the dry one with the queen and a small amount of brood out into the out world container, and shortly after the workers had all the brood moved into the newer test tube with the rest of their brood. For some reason the queen hasn't gone in the test tube, and instead seems to keep hanging around the same exact spot in the out world. It looks like workers are continually harassing her or trying to get her to go into the tube, I can't really tell which it is, I just know something doesn't seem right.
About a week and a half ago, I dug up another Dorymyrmex insanus queen from out in the Mojave Desert next to Phelan, California, where I saw a huge P. rugosus mating flight. This queen is way bigger than any other D. insanus queen I have ever seen, but I'm pretty sure that it is D. insanus. Being that D. insanus is actually more than one species, I guess this makes sense, as this could be different than the ones I found in the lower elevation mountains closer to where I live. Most of these queens, from what I have seen are around 6 mm in length, while this one is about 8.5 mm. So far she has a small pile of eggs, so we'll see what happens with her.
Here are some pictures showing the size difference.