The seperation of the brood is common, it is usually sorted by size/stage of brood. Pupae separate from large larvae, separate from small larvae, etc.
Sometimes queens can co-exist for quite some time, but with species that tend to be monogynous any big stresser might tilt the balance of power. Some of Drew's colonies had 50+ workers before they removed the extra queens.
One of my queens somehow wanted to attack the termite soldiers I fed her colony. She is part of a three queen Tetramorium colony I have, 25 workers. She was tussling with an aggressive soldier almost her size. Long story short, she must have got injured, because she was in the colony debris pile the next morning.
Never feed live termite soldiers to ants, unless your colony is massive and can handle casualties. Their mandibles are literally built for killing ants, so injuries are inevitable.
Edited by Myrmicinae, August 20 2015 - 4:20 PM.