I've moved both my 2 Formica Fusca colonies away from their 20ml syringes (I couldn't find test tubes anywhere around) when there were around 5 or 6 nanitics and both colonies are doing fine.
I've moved them because the syringes don't hold much water (that's my main criticism of them!). They were running dry of water, while already having some mold on the cotton, so I've decided to move them.
They are in acrylic (or plastic?) aquariums the size of my open hand, as outworlds. Both colonies moved very fast out of their syringes, in less than 24 hours. Little moisture and a very wide and exposed entranced might have encouraged them to move out quick and easy.
One colony has a formicarium attached (too big for them but they are ok) and with 5 nanitics they explore the outworld for food; and the other colony is inside a vinyl tube attached to a 2.5ml water syringe inside the outworld. They've locked themselves in with sand pebbles and barely come out but they seem to be ok inside working on their brood.
I have 5 more queens that are still waiting for their 1st nanitics and I will move them as soon as they are around 5 or 6. Not many and I would prefer a larger number of them, but their syringes are running out of water and in an outworld setup I can manage that better and not stress them, so I think this is the best compromise for them under these circumstances.
Edited by Formiga, August 28 2021 - 2:34 PM.