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And that is easy to do. In ants that perform pleometrosis and related social behaviors that will kill off every queen except one, just let them get pupae then you can separate before any chance at workers picking sides.
This is what I'm thinking of doing with CA02, except perhaps after letting them get workers first - because apparently they turn pleo from diapause, not before.
Past & Present
Veromessor pergandei, andrei, stoddardi; Novomessor cockerelli
Camponotus fragilis, Camponotus sansabeanus (inactive), vicinus, laevigatus/quercicola, CA02
Pogonomyrmex subnitidus, P. californicus (inactive)
Liometopum occidentale (inactive); Prenolepis imparis; Myrmecocystus mexicanus (inactive); Tetramorium sp. (inactive); Lasius sp.
Termites: Zootermopsis angusticollis, and a box of drywood termites that can't be seen
Isopods: (most no longer keeping) A. gestroi, granulatum, kluugi, maculatum, vulgare; C. murina; P. hoffmannseggi, P. haasi, P. ornatus; V. parvus, P. pruinosus, T. tomentosa
Spoods: (no longer keeping) Phidippus sp., other