Here are some of my advice:
Don't do everything based off one source of information. For example, watch videos and read threads from multiple people instead of just getting all of your ant keeping knowledge from one guy. I live in Florida right now and I've only been keeping ants here so the things I say might not apply to you at all times.
Be patient. This is something I struggle with. Ant keeping requires a lot of patience to be rewarding. I recommend checking on your founding queens once a week, she should have workers in less than two months. If you disturb you're queen too much, she might not lay any eggs or eat the eggs she laid. Keep her in a place that's dark and away from vibrations (off the ground).
Don't perform a risky action if not necessary, I did that a lot and I don't want you to make the same mistakes I've made. For example, don't attempt to move your ants out of their current nest unless necessary, it will stress them out. When I was a new ant keeper, I would always try to move my ants as soon as I see a little bit of mold, but it is completely unnecessary to do so, ants won't be harmed by a little bit of mold, only move your ants in situations such as the mold covering over half of the test tube water reservoir cotton.
Following up my last point, think thoroughly before performing an action. Before you removed the cotton and ended up accidentally destroying her eggs, were there anything else you could have done that had less chance of harming the queen or her brood? Would she really escape the test tube or were you just too worried? (Not saying you did the wrong thing I'm just setting possibilities.) When coming up with a solution to a problem, make sure to research and plan it thoroughly and think of alternatives and situations where your plan won't go so well and how to deal with it. Constantly moving your queen can harm her more than you think, when a queen settles down in an area and gets moved to a new area she cannot recognize, she can be seriously stressed out, but do not move your queen back now, try to keep her in the test tube you have her in right now unless she MUST move out of it. You can glue or tape cotton balls together to form stronger cotton balls that are harder for your queen to tear and escape through, or you can give her an out world so even if she manages to escape you would still know where she is. Make sure to keep the test tube wrapped in paper so it is always dark if you do provide her with an outworld. Example of an outworld can be a small tupperware big enough to fit her test tube with a hole bored out plugged with cotton for ventilation. Even if you're queen manages to obtain a way to leave her test tube it is unlikely she would actually leave the test tube as long as it is dark and humid because she already settled down and there is no other better place to nest.
Two eggs shouldn't be a big loss for her and if she laid eggs in only three days of you catching her she will likely lay more. Also don't worry about her dying, Camponotus are very hardy ants. You can try feeding her protein if you want, Camponotus are known to like fruit flies and crickets from pet stores. I saw a method of feeding cricket legs to ants without leaving the garbage in the test tube in a blog by TarHeelAnts, it demonstrated tying a string to the end of a cricket leg (not the thigh, the smaller part) and making sure the string is sticking out of the cotton that is blocking the entrance of the test tube while feeding so the caregiver can pull the cricket leg out after the ants are done eating. I have never tried this method but you shouldn't worry too much about leaving garbage in the test tube. You can also catch insects in the wild to feed the ants. I would recommend researching on what your species like to eat and sterilizing the insects somehow before feeding. Also make sure you feed dead food to your ants in founding, live food can seriously stress out small ant colonies.
Blog link: http://tarheelants.blogspot.com/
At this point I have realized that there are too many things about ant keeping I can't remember and write down. Don't worry too much about your queen, she will most likely be fine. If she does end up perishing you can always catch more. And if you have any more questions don't be afraid to ask!
Edited by super_oil, May 15 2018 - 8:52 AM.